Delimiting phylogeographic diversity in the genomic era: application to an Iberian endemic frog

  1. Dufresnes, Christophe 2
  2. Ambu, Johanna 2
  3. Galán, Pedro 9
  4. Sequeira, Fernando 3
  5. Viesca, Leticia 17
  6. Choda, Magdalena 1
  7. Álvarez, David 1
  8. Alard, Bérénice 3
  9. Suchan, Tomasz 6
  10. Künzel, Sven 5
  11. Martínez Solano, Iñigo 8
  12. Vences, Miguel 4
  13. Nicieza, Alfredo 17
  1. 1 Department of Biology of Organisms and Systems, University of Oviedo , C. Catedrático Rodrigo Uría s/n., 33006 Oviedo , Spain
  2. 2 Laboratory for Amphibian Systematic & Evolutionary Research, College of Biology and the Environment, Nanjing Forestry University , Longpan Rd, 159, 210037 Nanjing , People’s Republic of China
  3. 3 CIBIO, Centro de Investigação em Biodiversidade e Recursos Geneticos, Campus de Vairão, BIOPOLIS Program in Genomics, Biodiversity and Land Planning, Universidade do Porto , Rua Padre Armando Quintas, 7, 4485-661 Vairão , Portugal
  4. 4 Zoological Institute, Technische Universität Braunschweig , Mendelssohnstr, 4, 38106 Braunschweig , Germany
  5. 5 Max Planck Institute for Evolutionary Biology , August-Thienemann-Str, 2, 24306 Plön , Germany
  6. 6 W. Szafer Institute of Botany, Polish Academy of Sciences , Lubicz, 46, 31‑512 Kraków , Poland
  7. 7 Biodiversity Research Institute (IMIB), University of Oviedo-Principality of Asturias-CSIC , C. Gonzalo Gutiérrez Quirós s/n, 33600 Mieres , Spain
  8. 8 Museo Nacional de Ciencias Naturales, MNCN-CSIC , C. de José Gutiérrez Abascal, 2, 28006 Madrid , Spain
  9. 9 Grupo de Investigación en Bioloxía Evolutiva (GIBE), Departamento de Bioloxía, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira s/n., 15071 A Coruña , Spain
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Revista:
Zoological Journal of the Linnean Society

ISSN: 0024-4082,  1096-3642

Ano de publicación: 2023

Páxinas: 1-15

Tipo: Artigo

DOI: 10.1093/ZOOLINNEAN/ZLAD170 GOOGLE SCHOLAR lock_openAcceso aberto editor

Outras publicacións en: Zoological Journal of the Linnean Society

Resumo

The rich genetic and phenotypic diversity of species complexes is best recognized through formal taxonomic naming, but one must first assess the evolutionary history of phylogeographic lineages to identify and delimit candidate taxa. Using genomic markers, mitochondrial DNA barcoding, and morphometric analyses, we examined lineage diversity and distribution in the Iberian endemic frog Rana parvipalmata. We confirmed two deep phylogeographic lineages, one relatively homogenous genetically, found in Asturias and adjacent areas (T2), and one more fragmented and locally genetically impoverished, restricted to Galicia (T1). Analyses of their hybrid zone suggested a shallow transition characterized by far-ranging admixture, which was modelled by a wide geographic cline (~60 km for the genome average) and no obvious barrier loci (i.e. loci showing disproportionally restricted introgression). The relatively young T1 and T2 have thus remained reproductively compatible, which argues against their distinction as biological species, and we accordingly describe T2 as a new subspecies, Rana parvipalmata asturiensis ssp. nov. Remarkably, we highlight striking discordances between mitochondrial and nuclear distributions across their hybrid zones, as well as between their genetic and morphological differentiation. Our study illustrates how genomic-based phylogeographic frameworks can help decipher the high genetic and phenotypic variation of species complexes and substantiate the taxonomic assessment of candidate lineages.

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