In vivo reprogramming of ischemic brain as a new therapeutical way in the ischemic stroke

  1. López Loureiro, Ignacio José
Supervised by:
  1. José Antonio Castillo Sánchez Director
  2. Francisco Campos Pérez Co-director
  3. Tomas Sobrino Moreiras Co-director

Defence university: Universidade de Santiago de Compostela

Fecha de defensa: 07 February 2020

Committee:
  1. Isabel Rodríguez-Moldes Chair
  2. Mar Castellanos Rodrigo Secretary
  3. Alba Vieites Prado Committee member

Type: Thesis

Teseo: 607353 DIALNET lock_openMINERVA editor

Abstract

Ischemic stroke is the main cause of disability and an immense burden both on the resources of families and societies. Actual treatments are neuroprotective therapies only available for 3-7% of stroke patients. Consequently, new therapies are necessary to reduce the impact of ischemic stroke. In this Thesis we hypothesized that is possible to reprogram astrocytes to neurons using siRNAs for the inhibition of NOTCH1 pathway. This strategy could allow to reprogram astrocytes of the glial scar as a suitable treatment for the ischemic stroke. Therefore, we tested this treatment in an animal model of ischemic stroke. We also hypothesized that this treatment could be delivered in a tranlational way by the used of biodegradable non-toxic particles.