Ingeniería Tisular de la piel y del sistema nerviosoevaluación de nuevos soportes y fuentes de células

  1. Sanluis Verdes, Anahí
Supervised by:
  1. Nieves Doménech García Co-director
  2. María Esther Rendal Vázquez Co-director

Defence university: Universidade da Coruña

Fecha de defensa: 19 March 2018

Committee:
  1. Alexandre de la Fuente González Chair
  2. Silvia Díaz Prado Secretary
  3. Silvia Santos Cabrera Committee member
Department:
  1. Unknown

Type: Thesis

Teseo: 541333 DIALNET

Abstract

The healing process of wounds in adult humans leads to the formation of a scar, which does not completely restore the functionality of the damaged tissue and is also characterized by the loss of sensitivity and a poor aesthetic result. That is why we set out to develop two skin equivalents by Tissue Engineering using fibroblasts and keratinocytes that we obtained from skin biopsies and we have grown them on different supports such as the fibrin matrix obtained from plasma or the Amniotic Membrane (AM) decellularized obtaining good results. On the other hand, we studied the MA and the Umbilical Cord (UC) as an alternative source of stem cells since they can be easily isolated and cultured in vitro, with great capacity for proliferation, differentiation to the three embryonic lineages and cell-like cells nervous, being possible to synthesize an equivalent of nervous tissue if the latter are grown using MA as support.